N. Ichinose, S. Baba, T. Nakatsuji
Jun 18, 1993
Citations
0
Influential Citations
2
Citations
Journal
Fresenius' Journal of Analytical Chemistry
Abstract
SummaryA method is proposed for the determination of heat-stable alkaline phosphatase (HSAP) in serum. It comprises the following steps. The serum is heat-inactivated at 65°C and the residual alkaline phosphatase is allowed to react with Naphthol AS-MX phosphate. After stopping the reaction by acetone, the liberated Naphthol AS-MX is measured fluorometrically using high-performance liquid chromatography (HPLC). This method can be applied to cancer-screening in clinical examination. It is sensitive and rapid as compared with existing methods. Linearity, accuracy and detection limit have been examined (linearity 0–2190 ng Naphthol AS-MX; coefficient of variation ≈7%; recovery ≈80%).