K. H. Kumar, Sakina Razack, Ilaiyaraja Nallamuthu
2014
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0
Influential Citations
61
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Quality indicators
Journal
Industrial Crops and Products
Abstract
Abstract Cyperus rotundus is a traditional medicinal herb used in the treatment of various ailments. In the present study, we have evaluated in vitro anti-oxidant and free radical scavenging activities of 70% ethanolic, methanolic and water extracts of C. rotundus root. Among the extracts, the 70% ethanolic extract was found to be most potent with the IC 50 values of 64.64 ± 5.3 μg/mL, 85.89 ± 6.3 μg/mL and 8.42 ± 0.45 mg/mL against DPPH, metal chelating and nitric oxide scavenging activities and the observed activity could be correlated with metabolites such as polyphenols, flavonoids and sesquiterpenes identified by LC–ESI-MS/MS. Further C. rotundus was evaluated for its protective properties against macromolecules such as DNA, protein and lipid damage. The extract showed 48% protection against H 2 O 2 induced DNA damage and inhibited the AAPH and SIN-1 induced oxidation and nitration of BSA. Moreover, C. rotundus pretreatment restored the antioxidant status in white blood cells treated with H 2 O 2 . C. rotundus showed acetylcholine esterase inhibitory activity and the extract was found to be non-toxic up to 100 μg/mL in SH-SY5Y human neuroblastoma cell line. The ethanolic extract (200 mg/kg) also proved to be a potent anxiolyte as assessed by behavioural tests. Overall, the results suggest that this plant extract may be useful in combating oxidative stress related diseases through its antioxidant activity.