J. J. Koornstra, F. Rijcken, D. Jong
Jan 1, 2004
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Influential Citations
38
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Journal
Histopathology
Abstract
Aims : To investigate the monoclonal antibody M30 for the assessment of apoptosis in colorectal tissues. Although Terminal deoxyribonucleotidyl transferase mediated nick end labelling (TUNEL) and in‐situ end labelling (ISEL) are the methods most often used to demonstrate and quantify apoptosis in histological tissue sections, the interpretation and specificity of these techniques have been controversial. Immunohistochemistry using the monoclonal antibody M30 that recognizes caspase‐cleaved cytokeratin 18 is considered to be a promising alternative but has yet to be validated against a generally accepted standard.