Yong-Jin Lee, H. Yin, Young-ho Kim
Dec 1, 2004
Citations
0
Influential Citations
18
Citations
Quality indicators
Journal
Archives of Pharmacal Research
Abstract
Abstract6-Methoxydihydrosanguinarine (6ME), a benzophenanthridine alkaloid derived from the methanol extracts ofHylomecon hylomeconoides, showed a dose-dependent effect at 1–10 μM on causing apoptotic cell death in HT29 colon carcinoma cells (IC50 = 5.0 ± 0.2 μM). Treatment of HT-29 cells with 6ME resulted in the formation of internucleosomal DNA fragmentation. Treatment of the cells with 6ME caused activation of caspase-3, -8 and 9 protease and subsequent proteolytic cleavage of poly(ADP-ribose)polymerase. 6ME increased the expression of p53 and Bax and decreased the expression of Bid. These results indicate that p53 and proapoptotic Bcl-2 family proteins might participate in the antiproliferative activity of 6ME in HT29 cells.