R. Weinkam, J. Gal, P. Callery
1976
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Influential Citations
15
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Quality indicators
Journal
Analytical chemistry
Abstract
The stereoselective metabolism of the chiral psychotomimetic amine 1- (2,5-dimethoxy-4-methylphenyl)-2-aminopropane was studied using direct sample insertion isobutane chemical ionization mass spectrometric analysis of incubate extracts with the aid of deuterium labeled compounds as substrates and internal standards. Information was obtained on deuterium isotope effects, stereoselective substrate disappearance and metabolite formation. Mass spectrometric identification of 8 metabolites is reported for each enantiomer. Quantitative estimations of 16 incubate constituents was achieved by total ion current summation during the exhaustive evaporation of the incubate extracts into the ion source. The results suggest that direct sample insertion CIMS is an effective technique for the initial analysis of in vitro metabolic reactions. (auth)