Wu Yuan
2013
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Journal
Chinese Journal of Spectroscopy Laboratory
Abstract
The dyeing conditions for a fast protein staining method,combining microwaveheating with Coomassie Brilliant Blue G-250(CBB G-250),were investigated in SDS-PAGE.The whole procedure,which can be finished within 1 hour,is much faster than the conventional CBB R-250 staining protocol.No organic solvent and other acids but 3mmol/L hydrochloric acid was used in the method,which is benefical to operators and environment-friendly.Moreover,the obtained sensitivity was similar with that of the classic staining protocol.The method is applicable to the rapid analysis of protein electrophoresis in the experimental teaching and scientific research.