R. Lequin, H. Niall
Jan 26, 1972
Citations
0
Influential Citations
11
Citations
Journal
Biochimica et biophysica acta
Abstract
Abstract Pentafluorophenylisothiocyanate was used as a coupling agent in Edman degradation. All the pentafluorophenylthiohydantoins, except for arginine and histidine, could be identified by gas chromatography with the use of a one-column system. Their ultraviolet spectra and mobilities in thin-layer systems were studied. This new coupling agent was successfully used in short degradations of peptides and alternating with phenylthiocyanate in the degradation of a complex mixture of peptides of salmon calcitonin.