B. Garrett, A. L. Underwood
Mar 1, 1977
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Journal
Analytical biochemistry
Abstract
Abstract Extraction with ethyl acetate followed by gas-liquid chromatography on the extract provides a useful analytical method for phenol oxidase studies where it is desired to monitor directly the concentrations of volatile phenolic and catecholic substrates. A packed column with 10% DC-710 on Chromosorb W-AW-DMCS is recommended for such compounds as p -cresol, catechol, and 4-methylcatechol. p -Methoxyphenol is a suitable internal standard to minimize problems in the handling and injection of the extract. Examples of cresolase and catecholase kinetic runs are presented.