R. Amarowicz, M. Shimoyamada, K. Okubo
1991
Citations
0
Influential Citations
3
Citations
Journal
Nahrung-food
Abstract
Due to the high biological activity of saponins this group of compounds has become the object of interest of many researchers from different scientific disciplines [7]. In the analysis of saponins the stage of isolation of an investigated compound which follows the extraction from the plant material is decisive. Liquid column chromatography, e.g. ion-exchange chromatography [12], silica gelchromatography [1, 4, 5, 11], gel filtration on Sephadex LH-20 [6, 9, 10] and reversed phase chromatography have been used in the procedure. In the case of the latter columns were filled with octasilane [8] or octadecylsilane (ODS) bonded to silica gel [2, 4, 11]. The aim of the study was to apply reversed phase liquid chromatography in the analysis of saponins in faba bean.