John Eric Wilson
Oct 4, 1976
Citations
0
Influential Citations
122
Citations
Quality indicators
Journal
Biochemical and biophysical research communications
Abstract
Summary Cibacron Blue F3GA, the chromophore of Blue Dextran, interacted with all eight nucleotide-requiring enzymes that were examined. The conjugated chromophore (Blue Dextran) was more selective, interacting with enzymes including (but perhaps not restricted to) those known to possess the “dinucleotide fold”. The discriminating ability of these ligands should prove useful in elucidating the nature of nucleotide binding sites, and facilitate purification of selected nucleotide-requiring enzymes using affinity chromatography methods. Based on their comparable interactions with Cibacron Blue F3GA and weak interactions with Blue Dextran, it is suggested that neither yeast nor rat brain hexokinase contains the dinucleotide fold as a structural feature.