S. Creme, D. Leaback
Mar 15, 1980
Citations
0
Influential Citations
9
Citations
Journal
Analytical biochemistry
Abstract
Abstract A novel fluorogenic substrate (methylumbelliferyl 2-acetamido-2-deoxy-β- d -lactoside) has been prepared enzymatically. A procedure has been developed for its use as a convenient and sensitive fluorogenic substrate for β- d -galactosidase assay with a potential for high substrate specificity. The merits of this new fluorogenic substrate for β- d -galactosidase assays are discussed, together with the potential of this approach for a wider range of enzyme activities.