P. Pavithra, A. Mehta, R. Verma
Mar 15, 2018
Citations
1
Influential Citations
26
Citations
Quality indicators
Journal
Life Sciences
Abstract
Aims: Aromadendrene oxide 2 (AO‐(2)) is an oxygenated sesquiterpene naturally found as a chemical component of essential oils. In the present study anticancer activity of AO‐(2) has been investigated on A431 human epidermoid cancer and precancerous HaCaT cells. Material and methods: Cell viability was used to detect cytotoxic activity. Mechanism of cell death induced by AO‐(2) treatments was studied using Annexin V‐FITC/PI binding, cell cycle analysis, measurement of MMP and ROS generation by flow cytometry. Expression of apoptosis related proteins was investigated by western blot. Key findings: AO‐(2) inhibited the growth and colony formation ability of A431 and HaCaT cells in concentration dependent manner. It induced cell cycle arrest at G0/G1 phase and apoptosis through intracellular ROS accumulation. Inhibition of intracellular ROS by ascorbic acid and N‐acetyl cysteine treatment completely blocked apoptotic effect. N‐acetyl cysteine treatment significantly reversed G0/G1 arrest induced by AO‐(2). AO‐(2) treatment caused loss of mitochondrial membrane potential (&Dgr;&PSgr;m), increase in Bax/Bcl‐2 ratios, cytochrome c release, activation of caspases (cleaved caspase‐3 and caspase‐9) and PARP cleavage. AO‐(2) also significantly inhibited the growth of multicellular tumor spheroids of A431 and HaCaT cells. Significance: The results of the present study reveals that AO‐(2) a chemical component of essential oils induces apoptosis in A431 and HaCaT cells.