J. S. Byck, C. R. Dawson
1968
Citations
1
Influential Citations
35
Citations
Journal
Analytical biochemistry
Abstract
Abstract An experimental procedure, employing the techniques of column chromatography, has been developed by which the reactivity of waterinsoluble o -quinones toward protein can be evaluated semiquantitatively. This procedure has been used to study the effect of ring methylation on the ability of 3-pentadecyl- o -quinones to react to form protein conjugates. In this way it has been found that 3-pentadecyl- o -quinone is most reactive of all the compounds tested, whereas 4,5-dimethyl- and 4,5,6-trimethyl-3-pentadecyl- o -quinones, in which both of the normal sites for nucleophilic attack by the protein are blocked, do not react to any appreciable extent. The reactivity of all three monomethyl-3-pentadecyl- o -quinones has been found to be similar to that of the unblocked quinone, but the data for these compounds also indicate that there is some loss of reactivity resulting from steric interference by the lone methyl group in each case.