A. Kiszonas, C. Courtin, C. Morris
May 23, 2012
Citations
3
Influential Citations
34
Citations
Journal
Cereal Chemistry
Abstract
Arabinoxylans (AX) of wheat (Triticum aestivum L.) play a critical role in processing, end-use quality, and human health and nutrition. Consequently, an efficient, accurate method of AX quantification is desirable. The objective of this work was to evaluate a standard phloroglucinol colorimetric method for quantification of wheat AX. The method is based on the formation and spectrophotometric quantification of a phloroglucide product that results from the reaction of furfural produced during the condensation of pentose sugars with phloroglucinol. Method parameters, including reaction reagents and reaction times, were varied to identify areas for improved accuracy and consistency. Phloroglucide formation at three xylose concentrations was examined over time. The optimal reaction reagents and reaction times were determined based upon improved consistency in xylose quantification. The optimized method was used on xylose and arabinose standards and on whole meal wheat samples for total and water-extractable A...