Z. Chao
2013
Citations
0
Influential Citations
6
Citations
Journal
Chinese Journal of Appplied Environmental Biology
Abstract
The isolated microorganism Microbacterium oxydans C3 reduced 3,5-bis(trifluoromethyl)-acetophenone at 5 g/L to(R)-3,5-bis(trifluoromethyl)-1-phenethanol,a pharmaceutically alcohol intermediate for the synthesis of Aprepitant,a potent human NK-1 receptor antagonists,in 40 h.The bioreaction had desirable enantioselectivity( 99% enantiomeric excess [ee]) and 95% conversion.When the substrate concentration reached 50 g/L,the bioreaction afforded(R)-3,5-bis(trifluoromethyl)-1phenethanlo with 56% conversion and 99% ee.The usefulness of M.oxydans C3 was further demonstrated by the bioreduction of several fluorinated acetophenones with good conversion and excellent enantioselectivity.