C. Godinot
Sep 15, 1974
Citations
0
Influential Citations
5
Citations
Journal
FEBS Letters
Abstract
McGivan and Chappell reported in 1970 that avenaciolide has a specific inhibitory effect on glutamate transport in rat liver mitochondria [ 11. They observed 50% inhibition of glutamate oxidation on the addition of 47 I.~M avenaciolide [2] while oxidation of succinate, 3-hydroxybutyrate, citrate or malate was not inhibited by avenaciolide. They suggested that this inhibition was due to an analogy of structure between glutamate and avenaciolide and proposed to use this antibiotic to block glutamate transport specifically during investigations of the movements of other anions. On the basis of their conclusion, this antibiotic has been used for this purpose in many recent works [371. The present paper, shows that avenaciolide inhibits not only glutamate entry into mitochondria, but also entry of other dicarboxylic anions: for example, avenaciolide inhibits malate penetration when measured by the rate of reduction of endogenous pyridine nucleotide with a Ki lower than that measuredfor glutamate entry into rat liver mitochondria or pig heart mitochondria.