J. McKelvy, P. Leblanc, C. Laudes
Nov 22, 1976
Citations
4
Influential Citations
130
Citations
Quality indicators
Journal
Biochemical and biophysical research communications
Abstract
Bacitracin was found to be an effective inhibitor of the invitro degradation of both thyrotropin releasing factor1 (TRF) and luteinizing hormone releasing factor (LRF) by guinea pig hypothalamic and whole brain homegenates and rat hypothalamic homogenates and subcellular fractions. Bacitracin was effective in inhibiting the degradation of TRF and LRF, as determined by radioimmunoassay, where it exhibited no interference with the assays. Kinetic studies of the degradation of exogenous synthetic [3H]-TRF demonstrated non-competitive inhibition by bacitracin with Ki = 1.9 × 10−5 M, while studies on the degradation of [3H] LRF indicated competitive inhibition with Ki = 1.7 × 10−5 M. Electrophoretic and amino acid analysis revealed that bacitracin itself was not degraded during the course of the invitro incubation.