M. H. Li, Feifang Zhang, Hua Guo
Jun 15, 2010
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Clinical Oncology and Cancer Research
Abstract
Objective: To investigate the effects of the micro-molecular compound 4-methyl-3-nitro-benzoic acid on drug toxicity, migration capacity of various malignant tumor cells and inhibition of tumor growth on mice. Methods: The dosage of 4-methyl-3-nitro-benzoic acid was arranged by geometric series, and then the half lethal dose was measured on mice in vivo. Chemotaxis assay was performed to detect the effects of 4-methyl-3-nitro-benzoic acid on cell migration capacity of the lung cancer cells A549, liver cancer cells SSMC-7721, malignant melanoma cells LiBr and gastric cancer cells MGC-803 in vitro. In vivo, BL6-B16 cells were injected to C57 mice with normal sodium as the control. The effects of 4-methyl-3-nitro-benzoic acid on the growth of the mouse-transplanted tumors were observed after intra-gastric administration. The body weight of the mice was recorded as another measure to estimate the toxic effect of the drug, 4-methyl-3-nitro -benzoic acid. Results: It was shown by acute toxicity testing that the half lethal dose (LD50) of 4-methyl-3-nitro-benzoic acid in mice in vivo is 771.73 mg/kg. Chemotaxis assay showed that 4-methyl-3-nitro-benzoic acid could overtly and markedly inhibit the cell migration of many tumor cells with 2.0 μM as the optimal inhibiting concentration. 4-methyl-3-nitro-benzoic acid could inhibit the growth of mouse-transplanted melanoma, and the tumor volume (P0.05) and tumor weight (P 0.01) was markedly lower compared to the control group. 4-methyl-3-nitro-benzoic acid demonstrated no effect on body weight change in mice. Conclusions: 4-methyl-3-nitro-benzoic acid promises to be an effective new drug used in the treatment of malignant tumors.