Yong Zhang, Wei Li, Qiang Wang
2013
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0
Influential Citations
4
Citations
Journal
Acta Chimica Sinica
Abstract
A benzoxazole-based fluorescent probe 4-(benzo[ d ]oxazol-2-yl)-2-((bis(2-(ethylthio)ethyl)amino)methyl)phenol (ZY1) was designed and synthesized, and characterized by mass spectrometry (MS), nuclear magnetic resonance (NMR) and X-ray single crystal diffraction, etc . ZY1 was prepared by Mannich reaction, and its pale yellow crystals suitable for X-ray diffraction studies were obtained by slow evaporation of an ethyl acetate solution at room temperature over a period of a few days. The results showed that ZY1 exhibited good sensitivity and selectivity to Hg 2 + . The fluorescence of ZY1 was quenched dramatically in the presence of Hg 2 + , and it remained nearly constant in the range of pH 3.0 ~ 9.0. Other metal ions, such as K + , Ca 2 + , Na + , Mg 2 + , Mn 2 + , Fe 2 + , Fe 3 + , Co 2 + , Ni 2 + , Cu 2 + , Zn 2 + , Cd 2 + , Pb 2 + , induced negligible fluorescence quenching for ZY1 under the same conditions. When the concentration of Hg 2 + was increased from 0 to 30 µmol/L, the fluorescence of ZY1 (10 µmol/L) quenched gradually. Binding analysis using the method of continuous variations (Job’s plot) established that the stoichiometry of ZY1-Hg 2 + complex was estimated to be 1 ∶ 1. The fluorescence intensity varied almost linearly vs. the concentration of Hg 2 + (0.5 ~ 4.8 µmol/L), and the detection limit of Hg 2 + was estimated to be 0.08 µmol/L. Moreover, ZY1 was used for the visual detection of Hg 2 + and applied to image intracelluar Hg 2 + in living HeLa cells, indicating that it could be a useful tool in bioanalytical applications.