T. Ishii, T. Konishi, Yuki Ito
Oct 1, 2005
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Influential Citations
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Journal
Phytochemistry
Abstract
Arabinopyranosyltransferase (ArapT) activity that results in the transfer of a single arabinopyranose (Arap) residue from UDP-beta-L-arabinopyranose (UDP-Arap) to exogenous (1-->5)-linked alpha-L-arabino-oligosaccharides labeled with 2-aminobenzamide (2-AB) at their reducing ends was identified in a particulate preparation obtained from 3-day-old mung bean (Vigna radiate L. Wilezek) hypocotyls. The transferred Ara residue was shown to be beta-(1-->3)-linked to O-3 of the non-reducing terminal Araf residues of the oligosaccharide using nuclear magnetic resonance spectroscopy together with glycosyl composition and glycosyl linkage composition analyses. The 2AB-labeled arabino-octasaccharide was the most effective acceptor substrate analyzed, although arabino-oligosaccharides with a degree of polymerization between 4 and 7 were also acceptor substrates. Maximum ArapT activity was obtained at pH 6.5-7.0, and 20 degrees C in the presence of 25 mM Mn(2+) and 0.5% Triton X-100.