H. Sakamoto, Kazuya Watanabe, Ayako Koto
Jun 1, 2015
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Influential Citations
22
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Journal
Sensing and bio-sensing research
Abstract
Abstract l -hydroxyproline ( l -Hyp: trans -4-hydroxy- l -proline) is commonly found at high concentrations in connective tissue proteins such as collagen. It is a remarkably useful molecular marker because variation in the level of l -Hyp is associated with various diseases. Recently, the novel enzymes l -hydroxyproline epimerase and d -hydroxyproline dehydrogenase were isolated from bacteria. In this study, a novel electrochemical biosensor for l -Hyp was constructed using these two enzymes. l -hydroxyproline epimerase epimerized l -Hyp to d -hydroxyproline ( d -Hyp: cis -4-hydroxy- d -proline), and d -Hyp was oxidized with the reaction catalyzed by d -hydroxyproline dehydrogenase and mediated by ferrocene. We found that the sensor could determine l -Hyp concentrations of 10–100 μM with high-selectivity.