Y. Ogawa
Aug 1, 1968
Citations
2
Influential Citations
200
Citations
Quality indicators
Journal
Journal of biochemistry
Abstract
GEDTA (glycoletherdiaminetetraacetic acid, EGTA), a chelating agent which has a selective affinity for Ca ions compared with Mg ions, has greatly contributed to the clarification of the essential role of Ca ions in the contraction-relaxation cycle (1, 2). Since the concentration of Ca ions involved in physiological process is very low, i.e., not more than 10-5M, indirect measurement by means of a Ca-buffer is more feasible than direct measurement (2, 3). Because of its Ca specificity GEDTA is well suited for this purpose and has, therefore, been widely used in determining the concentration of Ca ions (2, 3). The binding constant of GEDTA used in these experiments was not determined