J. M. Holstein, Daniel Schulz, A. Rentmeister
Apr 1, 2014
Citations
2
Influential Citations
65
Citations
Journal
Chemical communications
Abstract
We present a chemo-enzymatic approach for site-specific labeling of 5'-capped RNAs based on bioorthogonal chemistry. A trimethylguanosine synthase was engineered to transfer a terminal azido moiety to the 5'-cap which could be further modified using strain-promoted azide-alkyne cycloaddition (SPAAC).