M. A. Ghalambor, E. Heath
Jul 10, 1966
Citations
2
Influential Citations
61
Citations
Journal
The Journal of biological chemistry
Abstract
Abstract Cytidine monophosphate 3-deoxy-D-manno-octulosonate synthetase catalyzes the reversible formation of cytidine monophosphate 3-deoxy-D-manno-octulosonate from cytidine triphosphate and 3-deoxy-D-manno-octulosonate. The synthetase has been purified approximately 170-fold from crude extracts of Escherichia coli 0111-B4. The purified enzyme exhibits an absolute requirement for a divalent cation (Mg++) and is specific for cytidine triphosphate (Km = 2.2 x 10-4 M) and 3-deoxy-D-manno-octulosonate (Km = 8 x 10-4 M).