Huai-zhi Zhang, Lijie Zhang, Xiaowei Yu
Feb 17, 2020
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0
Influential Citations
19
Citations
Journal
Journal of agricultural and food chemistry
Abstract
2-Ethyl-3,5(3,6)-dimethylpyrazine (EDMPs) has a pleasant aroma of roasted cocoa or nuts with extreme low odor threshold that have potential in industrial applications as food fragrances. Food fermentation process can accumulate EDMPs and that might be the chance to study the biosynthesis mechanism of EDMPs under mild conditions for "natural" EDMPs' production. In this study, an EDMP-producing strain was isolated from Baijiu fermentation. This strain was identified as Bacillus subtilis, a generally regarded as safe (GRAS) organism. After reasonable assumption, substrate addition and isotope-labelled experiments, we found EDMPs are produced from L-threonine and D-glucose at environmental temperature and pressure. In addition, aminoacetone, metabolite of L-threonine, and 2,3-pentanedione, metabolite of L-threonine and D-glucose, are intermediates for the production of EDMPs. This study proposed and confirmed the biosynthesis pathway of EDMPs. It will be helpful for the industrial production of EDMPs, and provides reference for the biosynthetic mechanism analysis of other valuable pyrazines.