M. Kajiwara, H. Hanamitsu, Masayuki Nakamura
2007
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Journal
Radioisotopes
Abstract
Culture broth of the photosynthetic bacterium Rhodobacter sphaeroides shows a strong red fluorescence. After esterification, two red fluorescent compounds were isolated and identified as uroporphyrin I octamethylester (minor) and coproporphyrin III tetramethylester (major) by FAB-MS, 1H-NMR and 13C-NMR. Feeding experiments using [2-13C] glycine indicated that, although the Shemin pathway was the main biosynthetic pathway, the coproporphyrin was enriched at all porphyrin carbons. Therefore, [2-13C] glycine appeared to have been incorporated into all porphyrin carbons via TCA cycle under anaerobic conditions.