D. Schachter, D. J. Kass, T. J. Lannon
1959
Citations
0
Influential Citations
52
Citations
Journal
The Journal of biological chemistry
Abstract
The biosynthesis of salicyl glucuronides in vitro provides a sensitive test for the capacity of tissues to form conjugates of glucuronic acid. The products of the conjugation are salicyl acyl glucuronide and salicyl phenolic glucuronide, for which specific and sensitive fluorimetric methods have been developed which detect as little as 2 mpmoles of each conjugate in the presence of a lOOO-fold excess of salicylate (2). The rates of conjugation of the carboxyl and the phenol groups of salicylate can be directly compared in these studies. It is well established that liver and kidney can form glucuronides (3). In addition, Hartiala (4) has reported that slices of rabbit, rat, and dog intestine conjugate o-aminophenol, although homogenates of the intestinal tract appear to be inactive (5, 6). The present studies with salicylate provide evidence that slices of the gastrointestinal tract, liver, kidney, urinary bladder, lung, and spleen are capable of glucuronide synthesis. It is of further interest that the salicyl glucuronides formed in the intestine can be accumulated in high concentration in the fluid bathing the serosal surfaces of everted gut-sacs.