M. Seegobin, A. Kisiala, Adam Noble
Jun 12, 2018
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Influential Citations
23
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Journal
The FASEB Journal
Abstract
Cytokinins (CKs) are a group of phytohormones essential to plant growth and development. The presence of these N6‐modified adenine derivatives has also been documented in other groups of organisms, including bacteria, fungi, and insects. Thus far, however, only a single CK, N6‐(Δ2‐isopentenyl) adenine‐9‐riboside (iPR), has been identified in mammals. In plants, the nucleotide form of isopentenyladenine [iPR (mono/di/tri)phosphate (iPRP)] is the first form of CK synthesized, and it is further modified to produce other CK types. To determine whether a similar biosynthesis pathway exists in mammals, we tested for the presence of 27 CKs in a wide selection of canine organs using HPLC electrospray ionization‐tandem mass spectrometry. Seven forms of CK were detected in the majority of the analyzed samples, including iPR, iPRP, cis‐zeatin‐9‐riboside, cis‐zeatin‐9‐riboside‐5′ (mono/di/tri)phosphate, 2‐methylthio‐N6‐isopentenyladenine, 2‐methylthio‐N6‐isopentenyladenosine, and 2‐methylthiozeatin. Total CK concentrations ranged from 1.96 pmol/g fresh weight (adrenal glands) to 1.40 × 103 pmol/g fresh weight (thyroid). The results of this study provide evidence that mammalian cells, like plant cells, can synthesize and process a diverse set of CKs including cis‐ and methylthiol‐type CKs.—Seegobin, M., Kisiala, A., Noble, A., Kaplan, D., Brunetti, C., Emery, R. J. N. Canis familiaris tissues are characterized by different profiles of cytokinins typical of tRNA degradation pathway. FASEB J. 32, 6575–6581 (2018). www.fasebj.org