D. Drocourt, Thierry Calmels, J. Reynes
Jul 11, 1990
Citations
6
Influential Citations
202
Citations
Quality indicators
Journal
Nucleic acids research
Abstract
Phleomycins are glycopeptide antibiotics related to bleomycins nucleotide sequence of the Sh ble gene and its polylinker and tallysomycins which are active at low concentrations on both fragments. prokaryotic and eukaryotic cells (1). We have cloned a phleomycin-resistance gene (Sh ble) from the genomic DNA of REFERENCES Streptoalloteichus hindustanus (ATCC 31158), a tallysomycin 1 Berdy,J. (1980) Handbook of.Antibiotic Compounds. Vol.4, pp. 459-491, producer. The Sh ble gene encodes a small acidic protein (MW CRC Press, Boca Raton, FL, USA 13665) which has been crystallised for X-ray structural studies 2. Rondeau,J.M., Cagnon,C., Moras,D. and Masson,J.M. (1989) J. MoI. Biol. (2). The Sh protein is a binding protein with a strong affinity 207, 645-646. for the phleomycin family of antibiotics (3). When these 3. Gatignol,A., Durand,H. and Tiraby,G. (1988) FEBS Lent. 230, 171-175. antibiotics are bound by the Sh protein, then phleomycins can 4. Glumoff,V., Kappeli,O., Fiechter,A. and Reiser,J. (1989) Gene 84, nolonger areactivated by tneffous ions and oxyen teobreakdcan 311-318. no longer be activated by ferrous ions and oxygen to break down 5. Mattern,J.E. and Punt,P.J. (1988) Fungal Genet. Newslett. 35, 25. DNA (3). The Sh ble gene has been used in a number of 6. Perez,P., Tiraby,G., Kallerhoff,J. and Perret,J. (1989) Plant Mol. Biol. 13, laboratories as a selectable marker for lower (4, 5) and higher 365-373. eukaryotes (6, 7, 8). In order to facilitate vector construction 7. Mulsant,P., Gatignol,A., Dalens,M. and Tiraby,G. (1988) Somat. Cell. Mol. exeukryoetes(, 8).theinordgoers wto fac tipltevctoricositruction Genet. 14, 243-252. periment , synthetic oligom with multiple cloning sites have 8. Cosset,F.L., Legras,C., Chebloune,Y., Savater,P., Thoraval,P., been added at both ends of the Sh ble gene to give the two pUC Thomas,J.L., Samarut,J., Nigon,U.M. and Verdier,G. (1990) J. Virol. 64, based derivatives, pUT56 and pUT58. We report here the 1070-1078.