W. Langgut
May 1, 1993
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0
Influential Citations
6
Citations
Quality indicators
Journal
BioFactors
Abstract
The guanine analogue, queuine (q), is a nutrition factor for eukaryotes and occurs as a free base or as modified nucleoside queuosine (Q) inserted into the anticodon of tRNAs(GUN) in place of guanosine (G). The free q-base and the corresponding Q-deficient tRNAs accumulate in fast proliferating tumors and in embryonic cells. The present data show that treatment of Q-deficient HeLa cells with queuine resulted in reduced in vitro phosphorylation of two membrane-associated proteins, pp110 and pp18. Reduced phosphorylation of pp110 and pp18 was also observed when a membrane-containing particulate fraction from queuine starved HeLa cells was labelled with [gamma-32P]ATP in the presence of queuine. Incorporated phosphate into pp110 was alkali-stable, suggesting that this protein became phosphorylated at tyrosine residues. After stimulation of intact Q-deficient cells with epidermal growth factor, pp110 was rapidly phosphorylated at tyrosine residues. The results show that queuine as a free base influences protein tyrosine phosphorylation in intact cells, suggesting that it might interfere with protein phosphorylation involved in signal transduction pathways.