Su Jinlon
2014
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Quality indicators
Journal
Journal of Practical Stomatology
Abstract
Objective: To investigate the effects of Bax channel blocker(BCB) on the biological characteristics of aged bone marrow stromal cells(aged-BMSCs). Methods: BMSCs from 22 month old(aged) and 2 week old rats(young) were obtained by whole bone marrow adherent culture and identified by β-gal staining. The optimal dose of BCB for the detection of Nanog and Oct-4 expression was determined by Real Time PCR. Then the senescence-associated characteristics at cellular and molecular levels and osteogenic ability were tested by RT-PCR and Western Blot. Osteogenic induction and BCB intervention were proceeded. ALP staining and Alizarin Red staining were conducted to observe the osteogenic potential of the cells. Results: BCB at 10 μmol/L(optional dose) declined β-gal expression in aged-BMSCs,decreased the expression of p53 and p21WAF1/cip1at mRNA and protein levels,and promoted the expression of Nanog and Oct-4,promoted the expression of osteogenesis-associated genes,increased ALP and Alizarin Red staining levels. Conclusion: BCB can reverse the biological characteristics of aged-BMSCs in vitro and promote the osteogenesis potential.