Kim Seung-Hwan, Song Seung-Hoon, Yoo Young-Je
2006
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0
Influential Citations
3
Citations
Journal
Journal of Microbiology and Biotechnology
Abstract
A gallocyanine-bound nanoporous titanium dioxide electrode system was investigated to carry out a mediated enzyme reaction. Gallocyanine was bound either directly or through an aminopropylsilane linker to the film of nanoporous titanium dioxide and used as a mediator for nitrate reductase in the mediated enzymatic nitrate reduction. The electrode with the aminopropylsilane linker showed 20% higher efficiency of electron transfer at the same potential than that directly linked. The prepared electrodes showed 0.26 μmol/h nitrate reduction at a 100 mm 2 surface of the electrode, and linear current response on nitrate ion concentration up to 1.0 mM, which is very useful as a biosensor of nitrate ion in water.