M. Philip, Abdul Khader Karakka Kal, M. Subhahar
Sep 20, 2021
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Influential Citations
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Quality indicators
Journal
Rapid communications in mass spectrometry : RCM
Abstract
RATIONALE Interest in growth hormone secretagogues has intensified during the past several years based on capable, ever-widening investigational applications of recombinant growth hormone in animals and humans. Ibutamoren is a potent, long-acting, selective, and orally-active non-peptide growth hormone secretagogue, which poses a great potential for abuse as a performance-enhancing agent in sports. METHODS To support drug metabolism and pharmacokinetic studies of chiral pharmaceuticals, it is necessary to combine the resolving power of HPLC with the sensitivity of mass spectrometric techniques. This paper describes the metabolic conversion of ibutamoren using equine liver microsomes and their characterization on a QExactive high-resolution mass spectrometer. RESULTS In this research, a total of thirty-two metabolites for ibutamoren (twenty phase I and twelve-phase II) were detected. The important findings of the current research are as follows: (1) the growth hormone secretagogue ibutamoren was prone to oxidation, results in corresponding hydroxylated metabolites; (2) in ibutamoren the dissociation of the phenyl ring and 2-amino-2-methyl- propanamide side chain also observed; (3) the glucuronic acid conjugates of mono, di and trihydroxylated analogues were detected; (4) no sulfonic acid conjugated metabolites were observed in this study of ibutamoren. CONCLUSIONS The reported data helps in the speedy detection of this growth hormone secretagogue ibutamoren and dissects its illegal use in competitive sports.