A. Tekin, Z. Dumlupinar, Adem Bardak
2022
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Journal
Genetika
Abstract
This research was carried out with the aim to detect the mutagenic effect of three different doses (15, 20 and 25 mM) of Ethyl Methane Sulphonate on the Ayval?k olive cultivar (Olea europaea L.), to detect the variation and to identify whether individuals are genetically different from Ayval?k olive cultivar using molecular marker technologies. 23 mutant lines were screened using 28 SSR markers and a total of 138 alleles were detected and the number of alleles per primer ranged from 2 to 9 with an average of 4.9 alleles per primer. The polymorphism information content (PIC) value was changed between 0.4-0.98. The genetic similarity between Ayval?k olive cultivar and mutant lines was found between 5.5% and 78%. The mutant lines 15mM-12, 15mM-11 and 25mM-3 were found similar to Ayval?k cultivars at the rate of 78, 63 and 55%, respectively. Whereas, 20mM-5, 15mM-4 and 15mM-15 mutant lines were found distant to Ayval?k cultivar at the rate of 5.5, 5.6 and 8% genetic similarity, respectively. Ethyl Methane Sulphonate was successfully used in this study to extend the genetic diversity of the Ayval?k olive cultivar. Mutants might be screened for agricultural and quality traits and promising lines might be registered.