Nannan Lu, He Liu, Rui Huang
Aug 20, 2019
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Influential Citations
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Journal
Analytical chemistry
Abstract
Researches about DNA composition have concentrated on DNA damage in the past few decades. However, it still remains a great challenge to construct a rapid, facile and accurate approach for simultaneously monitoring four DNA bases, guanine (G), adenine (A), thymine (T) and cytosine (C). Herein, a novel electrochemical sensor based on phenanthroimidazole deriv-ative, 2-(4-Bromophenyl)-1-phenyl-1H-phenanthro[9,10-d]-imidazole (PPI), is successfully fabricated by a simple electrochemical method. The bromophenyl group in PI could expand their aromatic plane, induce the π-conjugated extension and enhance the charge transfer and π-π interaction. The phenyl group at N1 position could regulate the intermolecular interaction, which could promote the possibility of intermolecular connection. The PPI polymer (poly(PPI)) with π-electron enriched conjugation architecture has been applied in simultaneous determination of G, A, T and C in neutral solution by square wave voltammetry (SWV) method with well-separated peak potentials at 0.714, 1.004, 1.177 and 1.353 V, respectively. The sensor functionalized with poly(PPI) exhibits wide linear response for G, A, T and C in the concentration ranges of 3-300 μM, 1-300 μM, 30-800 μM and 20-750 μM, respectively. With favorable selectivity, stability and reproducibility, the sensor is successfully utilized to monitor four DNA bases in real samples, displaying a promising prospect for electrochemical sensing devices.