P. Goland, N. G. Grand
Sep 1, 1968
Citations
0
Influential Citations
28
Citations
Journal
American journal of physical anthropology
Abstract
Cyanuric chloride (2,4,6-trichloro-s-triazine) and dichloro-s-triazinyl Procion M (Imperial Chemical) dyes and colorless compounds covalently bond or covalently cross-link with H-active functional groups in tissues including enamel, dentin, and bone. By cross-linking, chloro-s-triazines make irreversibly insoluble in situ various exogenous or endogenous components, which are lost or artifactually diffused by other methods of fixation. Cyanuric chloride (0.5%) and N-methyl morpholine (1%) in anhydrous methanol provide an excellent cytologic fixative, with N-methyl morpholine or other suitable tertiary amines serving as “quaternizing” catalysts and HCl acceptors. Highly permeable Procion M dyes were used topically as freshly prepared 5–10% aqueous solutions; 2% solutions in 5% dextrose were also given intravenously at a dosage level of 200 mgm/Kgm. Since topically applied dyes stain irreversibly, they can be employed as markers for the study of various pathophysiologic processes associated with the tooth and periodontal apparatus; that is, for the study of growth and development. Given intravenously, these dyes permanently mark incremental lines and zones of growth in tooth and bone; the markers persist during processing and decalcification of the specimens. Vinyl-sulfonyl Remazol (American Hoechst) and other, classes of reactive dyes were also used as permanent markers.