A. J. Sheppard, W. D. Hubbard
1971
Citations
0
Influential Citations
4
Citations
Journal
Methods in Enzymology
Abstract
Publisher Summary Menadione, a synthetic derivative with the physiological properties of vitamin K. Menadione and menadione sodium bisulfite, is currently included in many pharmaceutical products. Several water-soluble derivatives of menadione are currently in use, especially for animal feed fortification. These derivatives cannot be chromatographed per se, but, after precipitation with sodium hydroxide and extraction, the compound is recovered and chromatographed (GLC) as menadione. Studies have been made of the gas chromatographic behavior of menadione. Excellent analyses can be obtained for the menadione content of pharmaceutical tablets, capsules, and liquids. No significant difference between GLC results and the chemical method of the National Formulary has been found in laboratory. This chapter discusses the technique for preparation of packed columns for gas chromatography.