Y. Hatanaka, E. Yoshida, H. Nakayama
Dec 1, 1989
Citations
0
Influential Citations
12
Citations
Journal
Bioorganic Chemistry
Abstract
Abstract [[2-Nitro-4-[3-(trifluoromethyl)-3 H -diazirin-3-yl]]phenoxy]acetic acid and its derivatives have been synthesized as a special carbene precursor with a chromogenic group. Photolysis of the diazirine in methanol and cyclohexane gave intermolecular OH and CH insertion products, respectively. Spectroscopic properties of the diazirine derivatives and the photo-products revealed that irradiation and detection can be performed in a spectral region where the absorption due to most biological macromolecules is negligible. The application of this reagent will provide a useful approach for simple spectrophotometric detection of labeled products without recourse to conventional radioactive techniques in the photoaffinity labeling methodology.