H. Bundy
Sep 1, 1963
Citations
3
Influential Citations
67
Citations
Quality indicators
Journal
Archives of biochemistry and biophysics
Abstract
Both N-acetyl- and N-benzoyl-l-tyrosine p-nitroanilide serve as substrates for chymotrypsin. The benzoyl derivative provides the more sensitive substrate but requires the presence of organic solvent, while the acetyl derivative can be used in aqueous solution. Both substrates are slowly hydrolyzed by trypsin. Inhibition of the chymotrypsin-catalyzed hydrolysis by human blood serum has been investigated and values for Km(app), k3(app), and the Ki of indole have been determined at various acetone concentrations. The effect of acetone is discussed.