L. Barbas, M. Hamoy, V. J. Mello
Oct 1, 2017
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0
Influential Citations
44
Citations
Journal
Aquaculture
Abstract
Abstract The aim of this study was to evaluate the efficacy of essential oil of citronella, Cymbopogon nardus (EOC) as an anaesthetic for fish, using juvenile tambaqui Colossoma macropomum as a model. The times to induction and recovery after short-term anaesthesia were evaluated by concentration-response trials. Moreover, electrophysiological responses in muscle (EMG), electrocardiographic data (ECG), opercular beat intensity (OBI) and rate (OBR) were also evaluated. Juveniles were placed in aquaria containing six concentrations of EOC (200, 400, 600, 800, 1000 and 1600 μL L− 1) and the times of anaesthetic induction and recovery were registered. Sham control fish and fish exposed to ethanol-added water were used as controls. With the exception of the lowest concentration used (200 μL L− 1), deep anaesthesia was attained at all other subsequent concentrations, being the use of 600 μL L− 1 sufficient to promote rapid induction (