M. Jarman, F. Searle
Feb 15, 1972
Citations
0
Influential Citations
6
Citations
Journal
Biochemical pharmacology
Abstract
Abstract The chemical synthesis of 4-methylnicotinamide adenine dinucleotide from 3-carbamoyl-1-(2,3-O-isopropylidine- d -ribofuranosyl)-4-methyl pyridinium chloride is presented, and some properties of the modified coenzyme are described with particular reference to its potential as a cofactor for the glycolytic sequence in neoplastic cells. The compound was found to be amenable to chemical 1,4-reduction, to be capable of binding to glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12), and to act as a competitive inhibitor to nicotinamide adenine dinucleotide in the conversion of d -glyceraldehyde-3-phosphate to 1,3-diphospho- d -glycerate catalysed by this enzyme.