M. Iguchi, K. Mori, H. Ohyama
Aug 16, 1973
Citations
0
Influential Citations
4
Citations
Quality indicators
Journal
Biochimica et Biophysica Acta
Abstract
The mechanism of pyrrole-2-carboxylic acid formation from l-hydroxyproline was investigated using rat tissues. After a single administration of 200 mg l-hydroxy-proline, the urinary excretion of pyrrole-2-carboxylic acid remained strikingly elevated for 24 h. Pyrrole-2-carboxylic acid formation rates by rat kidney were studied in vitro. In this tissue, pyrrole-2-carboxylic acid formation was observed only under aerobic conditions. The pyrrole-2-carboxylic acid forming activity was primarily localized in the supernatant fraction obtained by centrifugation at 105 000 × g. In the purification steps used to obtain l-amino acid oxidase from the rat kidney, the activity of pyrrole-2-carboxylic acid formation directly increased as the l-amino acid oxidase activity increased. The ratio of the two activities was constant in each step of the purification of l-amino acid oxidase. From these results, it was concluded that l-hydroxyproline was oxidized to Δ′-pyrroline-4-hydroxy-2-carboxylic acid by l-amino acid oxidase and then converted to pyrrole-2-carboxylic acid spontaneously.