A. Szewczuk, M. Siewinski, R. Slowinska
Mar 15, 1980
Citations
1
Influential Citations
23
Citations
Journal
Analytical biochemistry
Abstract
Abstract All three isomers of phenylacetyl-aminobenzoic acid are stable in buffer solutions and are suitable as substrates for the simple and sensitive assay of penicillin amidase activity in purified preparations, in bacterial cells as well as in immobilized preparations. By titration of the active center with phenylmethylsulfonyl fluoride it was demonstrated that phenylacetyl-4-aminobenzoic acid as well as benzylpenicillin are hydrolyzed by the same enzyme. The new substrate was also used for rapid assay of the amidase activity in bacterial colonies. Both phenylacetyl-naphthylamides are very slowly hydrolyzed by the amidase.