N. Husain, Angela Y. Christian, I. Warner
May 5, 1995
Citations
0
Influential Citations
9
Citations
Journal
Journal of Chromatography A
Abstract
Abstract The effect of two co-modifier containing tert. -butyl moieties, tert. -butyl (N-hydroxy) carbamate and tert. -butyl carbazate, on the retention behavior of six polyaromatic hydrocarbons (PAHs) using cyclodextrin-modified mobile phases has been examined. Both modifiers resulted in shorter retention times for all PAHs, when a size-compatible cyclodextrin was present in the eluent. This decrease has been attributed to the formation of a ternary PAH-cyclodextrin-modifier complex. The apparent formation constants calculated for these complexes are of the order of approximately 10 2 M −1 , with the exception of the β-CD-pyrene complex, which was determined to have a 2:1 stoichiometry. Mixtures of β- and γ-cyclodextrins were used in the mobile phase along with tert. -butyl carbazate to achieve a baseline separation of all six PAHs under 50 min.