C. Atack, M. Lindqvist
2004
Citations
1
Influential Citations
81
Citations
Quality indicators
Journal
Naunyn-Schmiedeberg's Archives of Pharmacology
Abstract
SummaryProcedures for the fluorimetric assay of 5-hydroxytryptamine, 5-hydroxytryptophan and 5-hydroxyindole-3-acetic acid in tissues are described. The assay procedures are based both on the native fluorescence of 5-hydroxyindoles in strong acid and on the considerably higher fluorescence intensity of the fluorophores obtained by means of condensation with orthophthaldialdehyde. A tissue blank procedure, utilizing potassium ferricyanide and cysteine, common to both assays in the joint procedure, is incorporated. The assays have been adjusted to previously described chromatographic procedures, using a strongly acidic cation exchange column, in which these 5-hydroxyindoles and their precursor, tryptophan, and also other biogenic amines and related compounds, are isolated from a single Dowex 50 column. Data illustrating the reproducibility, sensitivity and increased specificity of the overall procedures compare favourably with previously published results. The possibility of the differential estimation of different 5-hydroxyindoles in a mixture, by means of simultaneous determinations of native and orthophthaldialdehyde-condensate fluorescence in the joint assays, is pointed out.