D. Umeno, M. Kawasaki, M. Maeda
Aug 20, 1998
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Influential Citations
41
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Journal
Bioconjugate chemistry
Abstract
Poly(N-isopropylacrylamide) having a terminal psoralen group was synthesized and covalently bound to plasmid pBR 322 DNA through a photochemical reaction. The resulting conjugate exhibited temperature-responsive precipitation due to the aggregation of poly(N-isopropylacrylamide) chains when heated above 31 degreesC. This system was used for the one-pot separation of restriction endonuclease EcoRI.