Zhang Jian-zhong
2010
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Journal
China Biotechnology
Abstract
1,5-pentanediamine is one of the most important industrial chemicals for its highly desired properties and its wide applications as a key component of an emerging polymer business. Biological production of 1,5-pentanediamine has been a novel and competitive way. An about 2.2 kb fragment of Hafnia alvei lysine decarboxylase gene encoding lysine decarboxylase (LDC) was proliferated by polymerase chain reaction by using chromosomal DNA of Hafnia alvei as the template. The obtained ldc fragment was inserted into E. coli/C. glutamicum shuttle vector pXMJ19 to construct an expression plasmid pXMJ19-ldc. It was then introduced into C.glutamicum TK260512 via electrotransformation, and a recombinant C.glutamicum TK260512/pXMJl9-ldc was obtained. The 1,5-pentanediamine in the broth was detected by HPLC when the culture in the shake flask was induced with 0.5 mmol/L IPTG. The results showed that after 36 h cultivation,the recombinant strain could produce 0.96 g/L 1,5-pentanediamine.