F. Alfani, M. Cantarella, N. Cutarella
Jun 15, 1992
Citations
0
Influential Citations
5
Citations
Journal
Journal of biotechnology
Abstract
The study deals with stability and activity of enzymes in supramolecular systems. Acid phosphatase (EC 3.1.3.2) has been studied as model enzyme. The organic phase is rich in C2-C4 acetates. Didodecyldimethylammonium chloride (DD-DACl) has been mainly used as ionic surfactants. The rate of enzyme inactivation is smaller than in buffer and is less dependent on storage temperature. Specific activity of the enzyme is lowered because of a less affinity towards the substrate and of reduction of maximal velocity.