S. Praveen Kumar, P. Srinivas, J. Rao
Apr 1, 2002
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Influential Citations
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Journal
JPC – Journal of Planar Chromatography – Modern TLC
Abstract
Rheum emodi is an important medicinal plant widely used in the Unani system of medicine. Asimple, precise and convenient HPTLC method has been developed for analysis of chrysophanol, an antioxidant and a major constituent of the rhizomes of Rheum emodi (Polygonacea) and pharmaceutical formulations of these. This method uses chrysophanol as internal standard. The stationary phase was silica gel 60F254, and hexane—ethyl acetate, 45 + 5 (v/v) was used as mobile phase. Detection and quantification were performed densitometrically at λmax = 366 nm. The method is characterized by high sensitivity and linearity over a wide concentration range. The results obtained were evaluated statistically.