S. Koritala, C. Hou, C. W. Hesseltine
Dec 1, 1989
Citations
4
Influential Citations
53
Citations
Journal
Applied Microbiology and Biotechnology
Abstract
SummaryResting cell suspensions of seven Nocardia species catalyzed the production of 10-hydroxystearic acid from oleic acid. Nocardia cholesterolicum NRRL 5767 gave a good yield with optimum conditions at pH 6.5 and 40°C. Yields exceeding 90% can be obtained within 6 h with 0.1 g cells (dry weight) and 178 mg oleic acid in 10 ml of 0.05 M sodium phosphate buffer (pH 6.5). In addition, minor amounts of 10-ketostearic acid were formed as a by-product. The reaction proceeded via hydration of the double bond as shown by labeling experiments with deuterium oxide and 18O-labeled water. The system was specific for fatty acids with cis unsaturation at the 9 position.